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Hitachi High Technologies America, Inc.

Hitachi

U-HPLC Systems | LaChromUltra

Hitachi LaChromUltra ultra high speed liquid chromatograph

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A complete U-HPLC system that delivers ultra high-speed performance without compromising flexibility, reliability, or ease of use.

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Hitachi's Ultra High-Speed Liquid Chromatograph, LaChromUltra,delivers ultra-fast analysis, higher resolution, increased sensitivity and the flexibility to run both conventional and ultra-high resolution LC analyses on a single system. LaChromUltra is the practical next generation LC solution that meets your laboratory's changing needs AND fits your budget.

Features:

  • 60 MPa (8700 psi) pressure max and 2.0 µm particle size columns result in ultra high-speed analysis (90% faster than conventional HPLC) and dramatically improved resolution.
  • Reduced system delay volume (as low as 266 µL) minimizes peak dispersion providing improved speed, resolution and sensitivity.
  • Reduced carryover (0.005%) improves precision, sensitivity and throughput.
  • Unique hand-tight column fitting system allows no-tools-required column changing making it very easy to change columns/methods.
  • Advanced Electronic Pulsation Isolation and Compensation (A-EPIC) provides consistent solvent flow reducing pulsation problems and improving gradient accuracy.
  • Improved detector response (10ms) ensures data integrity for ultra-narrow peak widths common in fast LC.
  • Advanced Gradient Function eliminates delay volume effect from gradient analysis improving system gradient performance and reducing analysis time.
  • Full Sequence Download Function streamlines Software/Auto Sampler interface resulting in faster cycle times and higher throughput.
  • Conventional and Ultra High-Speed LC analysis on a single system allows easy method transfer and the ultimate in flexibility.
  • Method Transfer Software Tool makes it easy to convert conventional HPLC methods to work on LCU.

For detailed information, please see the LaChromUltra brochure.

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Modules and Specifications

The LaChromUltra system consists of the following modules:

LCU System Only

  • L-2160U Pump (2 units)
  • L-2200U Autosampler
  • L-2300 or L-2350 Column Oven
  • L-2400U UV, L-2420U UV-Vis or L-2485U FL Detector
  • Organizer/Power Supply
  • Software: Empower™ 2
  • OS: Windows XP
  • Connection: USB

Specifications:

L-2160U Pump Specifications
Liquid feed system Dual-plunger reciprocating pump (series connection, pulsation suppression)
Flow rate range 0.001 to 5.000mL/min
Max. discharge pressure 60MPa (0.001 to 2.500mL/min)
Flow rate accuracy ±2% (0.101 to 2.500mL/min)
Flow rate precision RSD 0.07% or SD 0.02min
Dimensions/weight 340 (W) × 400 (D) × 150 (H)mm, 15kg
Power supply DC24V (supplied from organizer)
Power consumption 50 VA
Gradient System
Number of solutions mixable 2 solutions
Mixing system 2-pump flow rate control
L-2200U Autosampler Specifications
Number of samples 1.5mL × 200 pcs (1mL vial and micro plate usable with optional rack)
Sample injection system Direct injection
Sample injection volume 0.1 to 50µL
Injection volume reproducibility 0.3% RSD or less (when 10µL is injected)
Carryover 0.005% or less (under specified conditions)
Withstand pressure 60MPa
Sample cooling Thermoelectric cooling rack optionally available
Dimensions/weight 340 (W) × 400 (D) × 300 (H)mm, approx. 19kg
Power supply DC24V (supplied from organizer)
Power consumption 50 VA
L-2300 Column Oven Specifications
Range [Room temp - 15 °C] to[Room temp 50°C]
Setting range 1 to 65 °C
Accuracy ± 1 °C
Safety Built-in vapor/leak sensor
Stability ± 0.1 °C
Column capacity Up to three 25-cm columns
GLP functions Serial number registration in unit memory
Dimensions/weight

340 mm (W) x 400 mm (D) x 150mm (D)

approx. 10 kg
Power supply 100 V (50/60 Hz) (organizer/AC adapter not required)
Power consumption 200 VA
L-2350 Column Oven Specifications
Range [Room temp- 15 °C] to [Room temp 65 °C]
Setting range 1 to 85 °C
Accuracy ± 1 °C
Safety Built-in vapor/leak sensor
Stability ± 0.1 °C
Column capacity Up to four 50-cm columns (10 mm ID max)
GLP functions Serial number registration in unit memory
Options 3-column selector valve
6-way 2-position valve
10-way 2-position valve
Manual injector (Rheodyne 7725i)
Dimensions/weight

210 mm (W) x 360 mm (D) x 615 mm (H)

approx. 20 kg
Power supply 100V (50/60Hz) (organizer and AC adapter not required)
Power consumption 300VA
L-2400U UV Detector
Optical system Double-beam ratio photometry system
Light source D2 lamp, Hg lamp (for wavelength check)
Flow cell 3.2µL (optical path length 5mm)
Measuring wavelength range 190 to 600nm
Wavelength accuracy ±1nm
Noise 0.6 × 10–5 AU or less (250nm, air in cell, response 2.0sec, cell length 5mm)
Drift 1.0 × 10–4 AU/hr or less (250nm, air in cell, response 2.0sec, constant temperature, cell length 5mm)
Response 0.01 to 2.0sec (7 steps)
Data sampling period 10msec (shortest)
Dimensions/weight 340 (W) × 400 (D) × 150 (H)mm, approx. 11kg
Power supply DC24V (supplied from organizer)
Power consumption 85VA
L-2420U UV-Vis Detector
Optical system Double-beam ratio photometry system
Light source D2 lamp, Hg lamp (for wavelength check)
Flow cell 3.2µL (optical path length 5mm)
Measuring wavelength range 190 to 900nm
Wavelength accuracy ±1nm
Noise 0.6 × 10–5 AU or less (250nm/600nm, air in cell, response 2.0sec, cell length 5mm)
Drift 1.0 × 10–4 AU/hr or less (250nm/600nm, air in cell, response 2.0sec, constant temperature, cell length 5mm)
Response 0.01 to 2.0sec (7 steps)
Data sampling period 10msec (shortest)
Dimensions/weight 340 (W) × 400 (D) × 150 (H)mm, approx. 11kg
Power supply DC24V (supplied from organizer)
Power consumption 85VA
L-2485UFluorescence Detector
Light source Xe lamp, Hg lamp (for wavelength check)
Measuring wavelength range Excitation side: 200 to 850nm Emission side: 250 to 900nm (For 731nm or longer, the photomultiplier needs to be changed.)
Wavelength accuracy ±3nm
Spectral bandwidth Excitation side: 15nm Emission side: 15, 30nm (switchable)
Sensitivity S/N ratio at Raman peak of water: 550 or better [ultra high-speed LC flow cell: 3µL (volume irradiated)] (baseline method) (Em: 30nm) S/N ratio at Raman peak of water: 900 or better (standard flow cell)(baseline method)(Em: 30nm)(option)
Material of wetted parts Quartz, fluorocarbon resin
Response 0.01 to 2.0sec (7 steps)
Data sampling period 10msec (shortest)
Dimensions/weight 340 (W) × 400 (D) × 300 (H)mm, approx. 23kg
Power supply 100 V (50/60 Hz) (organizer and AC adapter not required)
Power consumption 450VA
Organizer
Output DC24V, 300VA Power supply to 2 pumps, 1 auto sampler and 1 detector
Dimensions/weight 340 mm (W) x 400 mm (D) x 150mm (D), approx.10 kg
Power supply 100 V (50/60 Hz)
Power consumption 400 VA

LaChromUltra Technology

Why Ultra High-Speed LC?

LaChromUltra uses 2.0 μm particle size columns resulting in dramatically higher column efficiency compared to conventional HPLC systems. When using ≤ 2.0 μm particle sizes columns, efficiency does not diminish with increased linear velocity—resulting in both increased analysis speed and higher resolution.

Van Deemter Plot

Vertical axis: theoretical plate height (Chromatographic Efficiency)
Column length/theoretical plate number -- smaller number indicates higher column performance

Horizontal axis: linear velocity (Flow rate in the column)
The optimal flow rate range for 5 μm particle is narrow while the theoretical plate height for 2 μm particle remains very consistent at increased flow rates.

As a result, the analysis time is reduced by 90% compared to our conventional HPLC system.

LCU Result Comparison

 

 

Application Notes

Literature

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