HPLC Pump | L-2130 HTA

The LaChrom Elite Solvent Delivery Modules deliver highly reproducible results over a wide range of flow rates. Compatible with both 1-mm micro-bore and high-throughput applications, as well as conventional columns, LaChrom Elite Pumps set new standards for flexibility, accuracy, and reliability in both isocratic and gradient modules of operation. As part of the LaChrom Elite HPLC system, these pumps are designed to meet your most stringent requirements, including government requirements for documentation and system suitability, while providing maximum up time and the lowest cost of ownership.

Binary high-pressure mixing or quaternary low-pressure mixing modes of operation.
Built-in time events allow contact closure of external devices such as column switching valves.
High-resolution stepping motors and piston diameters matched to the flow rate allow the lowest mixing columns in high pressure mode.

L-2130 HTA Pump Specifications
Accuracy	+/- 2 µL (0.01 to 0.10 mL/min) +/- 2 % (0.101 to 8.000 mL/min) +/- 4 % (8.001 to 9.999 mL/min)
Precision	+/-0.075 % RSD or 0.02 min SD at 1.0 mL/min
Constant pressure	290 to 5600 psi at 0 to 5.0 mL/min
Solvent delivery	100 µL stroke
Dimensions	13.5 in (W) x 15.75 in (D) x 6.0 in (D) (340 mm (W) x 400 mm (D) x 150 mm (D))
Weight	approx. 24 lbs (11 kg)
HIGH PRESSURE GRADIENT
Flow rate range	0.001 to 9.999 mL/min (0.2 to 5.0 mL/min recommended)
Binary	Up to 2 eluents
Composition range	1 to 100% (0.1% increments)
Accuracy	+/- 1 % (0.2 mL/min)
Precision	0.1% RSD or 0.02 min SD at 1.0 mL/min
LOW PRESSURE GRADIENT
Flow rate range	0.001 to 9.999 mL/min (0.2 to 5.0 mL recommended)
Quarternary	Up to 4 eluents
Composition range	0 to 100% (0.1% increments with CDS; 1% increments with front panel control
Accuracy	+/- 1 % (0.2 mL/min)
Precision	0.1 % RSD or 0.02 min SD at 1.0 mL/min

Application Notes

Amino Acid Analysis via the Hitachi Sensivate Elite Post-Column Reactor and LaChrom Elite HPLC

Note Number: 20100209

Amino acid analysis is an important technique involved in a wide range of bioanalytical applications including protein and peptide analysis, quality assessment of foodstuffs and clinical diagnosis of metabolic disorders. Historically, this technique has been performed on highly specialized chromatographic systems dedicated for comprehensive (up to 50-plus components) and quantitative amino acid analysis.

Amino Acid Analysis via PCR & HPLC (size 135.57 KB)
Automated Method Development System: Polar Reversed Phase Separations

Tranditional HPLC method development involves highly skillful, exerienced, knowledgeable, and intuitive operators to generate efficient separation methods. This is often a time-consuming endevor that does not always produce the best possible separation results in spite of the best efforts. Automated method development (AMD) with the Hitachi LaChrom Elite (LCE) HPLC system in conjunction with ChromSword Auto (CSA) provides a unique AMD system that will separate multi-component systems effortlessly, accurately, and with significant savings in both time and costs for the methoddevelopment.

LaChrom_Elite_App_Note_2_Sept03.pdf (size 158.91 KB)
Fast LC Pharmaceutical Applications: OTC Analgesics & Steroids

Routine development of multi-component 'Fast Liquid Chromatography' (Fast LC) applications are rapidly and easily accomplished by integrating (a) the new Hitachi LaChrom Elite HPLC system, (b) Automated Method Development tools (ChromSword AUTO), and (c) Hitachi Chromolith monolithic reversed-phase columns. These Fast LC applications allow for high-throughput analyses leading to a significant reduction in run times contrasted to conventional C18 particulate column run times by several-fold.

LaChrom_Elite_App_Note_1_Feb03(1).pdf (size 100.09 KB)
HPLC Analysis of Polyaromatic Hydrocarbons with DAD Detection

The analysis of polyaromatic hydrocarbons is shown using the highly flexible Hitachi LaChrom Elite HPLC system with diode array detection.

HPLC Analysis of Polyaromatic Hydrocarbons with DAD Detection (size 158.61 KB)
Simultaneous HPLC Analysis of Quercitrin, Isoquercitrin, and Hyperoside

Note Number: 20090807

Simultaneous Analysis of Quercitrin, Isoquercitrin, and Hyperoside on the Hitachi LaChrom Elite® Liquid Chromatography System

This application note describes the simultaneous analysis of three flavinoid compounds in the quercetin family. The peaks are well-resolved, and the method shows excellent reproducibility, linearity, and system suitability for the three analytes. The analysis was accomplished on a Hitachi LaChrom Elite® Liquid Chromatography System equipped with a Phenomenex Luna (2) column.

20090807-Hitachi Quercitrins Application Note-sec.pdf (size 401.32 KB)
Sorbitol Analysis Using HPLC with Refractive Index Detection

Note Number: 20091101

Analysis of Sorbitol in Apples on the Hitachi LaChrom Elite® Liquid Chromatography System Using Refractive Index Detection

Sorbitol Analysis Using HPLC with Refractive Index Detection (size 207.47 KB)
Vitamin E Analysis Using HPLC & ELSD

Note Number: 20091001

Analysis of Vitamin E Acetate on the Hitachi LaChrom Elite® Liquid Chromatography System Using the SofTA 400 ELSD Detector

Vitamin E (tocopherol) is a common antioxidant formulated into topical remedies. The acetate form of alpha-tocopherol is used as a standard analyte for the evaporative light scattering detector (ELSD). The goal of this study was to show reproducibility and suitability in detecting Vitamin E Acetate on the Hitachi LaChrom Elite® liquid chromatography system using the SofTA 400 ELSD detector.

Vitamin E Analysis Using HPLC & ELSD (size 301.67 KB)

Literature

Brochure | LaChrom Elite HPLC System

The LaChrom Elite® HPLC System provides precision, flexibility and reliability for the most demanding HPLC applications, setting a new standard in HPLC.

LaChrom Elite HPLC Brochure (size 502.67 KB)

HPLC Pump | L-2130 HTA

Application Notes

Literature

Liquid Chromatography

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