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Hitachi High Technologies America, Inc.

Hitachi

HPLC Detector | L-2400 UV/L-2420 UV-Visible

UV and UV-Visible Detectors for LaChrom Elite HPLC

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Superior signal-to-noise even at the fastest response times and a selection of flow cells

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Advanced optics and active electronics combine in the LaChrom Elite® L-2400/L-2420 Detectors to provide a new level of performance for UV and UV/Vis detection. Pre-aligned flow cell options accessed from the front of the instrument allow the user to easily switch from micro-bore to conventional or high throughput. Active noise filters with a wide range of response times produce superior signal-to-noise while maintaining peak shape in high flow rate applications. Automatic wavelength calibration and documentation within Empower™ 2 software make it easy to comply with government regulations for system suitability.

To improve light intensity and detection sensitivity in the visible range, the L-2420 UV/Vis Detector contains a tungsten lamp in addition to the deuterium lamp.

  • Available with a wide range of flow cell
  • External inputs for lamp off switching and auto zero
  • Variable signal output for best peak presentation
  • Spectrum recording with baseline subtraction for comparisons

*The photo shown above is Model L-2400 UV Detector. Empower™2 is a trademark of Waters Corporation.

L-2400 UV/L-2420 UV/VisDetectors Specifications
Optics Double beam and ratio mode
Calibration Built-in Hg lamp
Range L-2400: 190 to 600 nm
L-2420: 190 to 900 nm
Light source L-2400: D2 lamp
L-2420: D2 lamp and W lamp
Accuracy +/- 1 nm
Bandwidth 6 nm
Noise < 0.6 x 10-5 AU
Drift < 1.0 x 10-4 AU/h
AUFS range 0.25, 0.5, 1, 2 AU
Response 0.05, 0.1, 0.5, 1, 2, 4, 8 s
Programs 9 programmable steps
Max. Time 600 min
Max. Steps 100 (in 9 programs)
Available functions Wavelength, time, autozero, and hold
GLP functions Auto calibration using Hg lamp
Lamp energy check
Log for usage
Replacement date
Serial number registration in unit memory
Safety Bult-in leak sensor, Error handling
Dimensions 13.5 in (W) x 15.75 in (D) x 6.0 in (D)
(340 mm (W) x 400 mm (D) x 150mm (D))
Weight approx. 24 lbs (11 kg)

 

 

Application Notes

  • Amino Acid Analysis via the Hitachi Sensivate Elite Post-Column Reactor and LaChrom Elite HPLC
    20100209

    Amino acid analysis is an important technique involved in a wide range of bioanalytical applications including protein and peptide analysis, quality assessment of foodstuffs and clinical diagnosis of metabolic disorders.  Historically, this technique has been performed on highly specialized chromatographic systems dedicated for comprehensive  (up to 50-plus components) and quantitative amino acid analysis.

    application/pdf icon
    Amino Acid Analysis via PCR & HPLC (size 135.57 KB)
  • Analysis of Parabens Using the Prep-24 Dual Piston Pump with an Analytical-Scale HPLC System
    20090329

    Using the Prep-24 Dual Piston Pump System for Analysis of Parabens with the Hitachi LaChrom Elite® Liquid Chromatography System

    The Prep-24 pump manufactured by Scientific Systems, Inc. was used in analytical mode, along with the Hitachi HPLC system, to analyze a four-component mixture of parabens. The Prep-24 system, running in analytical mode, showed data that was suitable, reproducible, and linear.

    application/pdf icon
    Analysis of Parabens Using the Prep-24 Dual Piston Pump with an Analytical-Scale HPLC System (size 281.92 KB)
  • Automated Method Development System: Polar Reversed Phase Separations

    Tranditional HPLC method development involves highly skillful, exerienced, knowledgeable, and intuitive operators to generate efficient separation methods. This is often a time-consuming endevor that does not always produce the best possible separation results in spite of the best efforts. Automated method development (AMD) with the Hitachi LaChrom Elite (LCE) HPLC system in conjunction with ChromSword Auto (CSA) provides a unique AMD system that will separate multi-component systems effortlessly, accurately, and with significant savings in both time and costs for the methoddevelopment.

    application/pdf icon
    LaChrom_Elite_App_Note_2_Sept03.pdf (size 158.91 KB)
  • Fast LC Pharmaceutical Applications: OTC Analgesics & Steroids

    Routine development of multi-component 'Fast Liquid Chromatography' (Fast LC) applications are rapidly and easily accomplished by integrating (a) the new Hitachi LaChrom Elite HPLC system, (b) Automated Method Development tools (ChromSword AUTO), and (c) Hitachi Chromolith monolithic reversed-phase columns. These Fast LC applications allow for high-throughput analyses leading to a significant reduction in run times contrasted to conventional C18 particulate column run times by several-fold.

    application/pdf icon
    LaChrom_Elite_App_Note_1_Feb03(1).pdf (size 100.09 KB)
  • Simultaneous HPLC Analysis of Quercitrin, Isoquercitrin, and Hyperoside
    20090807

    Simultaneous Analysis of Quercitrin, Isoquercitrin, and Hyperoside on the Hitachi LaChrom Elite® Liquid Chromatography System

    This application note describes the simultaneous analysis of three flavinoid compounds in the quercetin family. The peaks are well-resolved, and the method shows excellent reproducibility, linearity, and system suitability for the three analytes. The analysis was accomplished on a Hitachi LaChrom Elite® Liquid Chromatography System equipped with a Phenomenex Luna (2) column.

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    20090807-Hitachi Quercitrins Application Note-sec.pdf (size 401.32 KB)

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